RNF121 Antibody

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ProSci
Product Code: PSI-8285
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8285-0.02mg0.02mg£150.00
Quantity:
PSI-8285-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
RNF121 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.
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Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.

Documents

Further Information

Additional Names:
Ring finger protein 121
Application Note:
RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 10 μg/ml.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1). It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK). Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1).
Background References:
  • Darom A, Bening-Abu-Shach U, and Broday L. RNF-121 is an endoplasmic reticulum-membrane E3 ubiquitin ligase involved in the regulation of B-integrin. Mol. Biol. Cell 2010; 21:1788-98.
Buffer:
RNF121 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
RNF121 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RNF121.

The immunogen is located within amino acids 260 - 310 of RNF121.
NCBI Gene ID #:
552978
NCBI Official Name:
ring finger protein 121
NCBI Official Symbol:
RNF121
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 24, 27, 36 kDa

Observed: 28 kDa
Protein Accession #:
NP_060790
Protein GI Number:
21361732
Purification:
RNF121 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist.
Swissprot #:
Q9H920
User NOte:
Optimal dilutions for each application to be determined by the researcher.