VTI1a Antibody
| Code | Size | Price |
|---|
| PSI-8287-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8287-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
VTI1a antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Vesicle transport through interaction with t-SNAREs 1A, MMDS3, MVti1, VTI1RP2, Vti1-rp2
Application Note:
VTI1a antibody can be used for detection of VTI1a by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). VTI1a mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane and promotes fusion of the lipid bilayers (4). VTI1a may be concerned with increased secretion of cytokines associated with cellular senescence (5).
Background References:
- Fischer VM and Stevens TH. A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways. J. Biol. Chem. 1998; 273:2624-30.
- Kreykenbohm V, Wenzel D, Antonin W et al. The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. Eur. J. Cell Biol. 2002; 81:273-80.
- Antonin W, Riedel D, von Mollard GF, et al. The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. J. Neurosci. 2000; 20:5724-32.
- Atlashkin V, Kreykenbohm V, Eskelinen EL, et al. Deletion of the SNARE vti1b in mice results in the loss of a single SNARE partner, syntaxin 8. Mol. Cell Biol. 2003; 23:5198-207.
Buffer:
VTI1a antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
VTI1a antibody was raised against a 19 amino acid peptide near the center of human VTI1a.
The immunogen is located within amino acids 50 - 100 of VTI1a.
The immunogen is located within amino acids 50 - 100 of VTI1a.
NCBI Gene ID #:
143187
NCBI Official Name:
vesicle transport through interaction with t-SNAREs 1A
NCBI Official Symbol:
VTI1a
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 24, 27 kDa
Observed: 26 kDa
Observed: 26 kDa
Protein Accession #:
NP_660207
Protein GI Number:
113374156
Purification:
VTI1a antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
VTI1a antibody is human, mouse and rat reactive. At least two isoforms of VTI1a are known to exist; this antibody will detect both isoforms. VTI1a antibody is predicted to not cross-react with VTI1b.
Swissprot #:
Q96AJ9
User NOte:
Optimal dilutions for each application to be determined by the researcher.