BTK (phospho Tyr223) Antibody

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ProSci
Product Code: PSI-79-779
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-779-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from Hela cells treated with serum using BTK (phospho-Tyr223) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from Hela cells treated with serum using BTK (phospho-Tyr223) Antibody. The lane on the right is treated with the antigen-specific peptide.

Documents

Further Information

Additional Names:
AGMX1, ATK, kinase Btk, BPK
Application Note:
Western Blot: 1:500~1:1000
Background:
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.
Background References:
  • Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676.
  • Yan M. Li, Cancer Res., Nov 2003; 63: 7630 - 7633.
  • Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443 - 449.
  • Feng Dong, Infect. Immun., Mar 2005; 73: 1861 - 1864.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BTK (phospho Tyr223) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 223 (A-L-Y (p)-D-Y) derived from Human BTK.
Modifications:
Phospho-Specific
NCBI Gene ID #:
695
NCBI Official Name:
Bruton agammaglobulinemia tyrosine kinase
NCBI Official Symbol:
BTK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
NP_000052.1
Protein GI Number:
547759
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of BTK only when phosphorylated at tyrosine 223.
Swissprot #:
Q06187