FOXO1/3/4-pan (phospho Thr24/32) Antibody

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ProSci
Product Code: PSI-79-788
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-788-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from 293 cells treated with Serum using FOXO1/3/4-pan (Phospho-Thr24/32) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from 293 cells treated with Serum using FOXO1/3/4-pan (Phospho-Thr24/32) Antibody. The lane on the right is treated with the antigen-specific peptide.

Documents

Further Information

Additional Names:
FKHR, FOXO1, FKHR2, AFX, MLLT7
Application Note:
Western Blot: 1:500~1:1000
Background:
Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.
Background References:
  • Robert J. Southgate, J. Biol. Chem., Jul 2007; 282: 21176 - 21186.
  • Susumu Kodama, Mol. Cell. Biol., Sep 2004; 24: 7931 - 7940.
  • Dragan Marinkovic, J. Clin. Invest., Aug 2007; 117: 2133 - 2144.
  • Marie-Liesse Asselin-Labat, Blood, Jul 2004; 104: 215 - 223.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FOXO1/3/4-pan (phospho Thr24/32) antibody was raised against a peptide sequence around phosphorylation site of threonine 24/32 (S-C-T (p)-W-P) derived from Human FOXO1/3/4-pan .
Modifications:
Phospho-Specific
NCBI Gene ID #:
2308/4303
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
78 kDa
Protein Accession #:
NP_002006.2
Protein GI Number:
9257222
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of FOXO1/3/4-pan only when phosphorylated at threonine 24/32.
Swissprot #:
Q12778/O43524/P98177