Pim-1 (phospho Tyr309) Antibody
| Code | Size | Price |
|---|
| PSI-79-800-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1, EC 2.7.11.1
Application Note:
Western Blot: 1:500~1:1000
Background:
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.
Background References:
- Selten G., Cell 46:603-611(1986).
- Saris C.J., EMBO J. 10:655-664(1991).
- Maita H., Eur. J. Biochem. 267:5168-5178(2000).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Pim-1 (phospho Tyr309) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 309 (A-A-V (p)-W-S) derived from Human Pim-1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5292
NCBI Official Name:
pim-1 oncogene
NCBI Official Symbol:
PIM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
NP_001230115.1
Protein GI Number:
83305339
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.
Swissprot #:
P06803