PKC alpha (phospho Tyr658) Antibody
| Code | Size | Price |
|---|
| PSI-79-806-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
PKC-alpha, KPCA, PKC III, PKC-A
Application Note:
Western Blot: 1:500~1:1000
Background:
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells.
Background References:
- Finkenzeller G., Nucleic Acids Res. 18:2183-2183(1990).
- McSwine-Kennick R.L., J. Biol. Chem. 266:15135-15143(1991).
- Gevaert K., Nat. Biotechnol. 21:566-569(2003)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PKC alpha (phospho Tyr658) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 658 (F-S-Y (p)-V-N) derived from Human PKC alpha.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5578
NCBI Official Name:
protein kinase C, alpha
NCBI Official Symbol:
PRKCA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
NP_002728.1
Protein GI Number:
317373571
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of PKC alpha only when phosphorylated at tyrosine 658.
Swissprot #:
P17252