AML1 (phospho Ser435) Antibody
| Code | Size | Price |
|---|
| PSI-79-893-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
Acute myeloid leukemia 1 protein, CBFA2, CBFA2, alpha 2 subunit, RUNX1
Application Note:
Western Blot: 1:500~1:1000
Background:
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
Background References:
- Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
- Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).
- Nucifora G., Blood 81:2728-2734(1993).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
AML1 (phospho Ser435) antibody was raised against a peptide sequence around phosphorylation site of Serine 435 ( S-N-S (p)-P-T) derived from Human AML1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
861
NCBI Official Name:
runt-related transcription factor 1
NCBI Official Symbol:
RUNX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
NP_001001890.1
Protein GI Number:
223459612
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.
Swissprot #:
Q01196