PLD1 (phospho Thr147) Antibody

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ProSci
Product Code: PSI-79-927
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-927-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HepG2 cells using PLD1 (Phospho-Thr147) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from HepG2 cells using PLD1 (Phospho-Thr147) Antibody. The lane on the right is treated with the antigen-specific peptide.

Documents

Further Information

Additional Names:
PLD 1, hPLD1, Choline phosphatase 1
Application Note:
Western Blot: 1:500~1:1000
Background:
Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.
Background References:
  • Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646].
  • Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774].
  • Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PLD1 (phospho Thr147) antibody was raised against a peptide sequence around phosphorylation site of threonine 147 (R-H-T (p)-F-R) derived from Human PLD1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5337
NCBI Official Name:
phospholipase D1, phosphatidylcholine-specific
NCBI Official Symbol:
PLD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
120 kDa
Protein Accession #:
NP_002653.1
Protein GI Number:
4505873
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.
Swissprot #:
Q13393