PKCB (phospho Ser661) Antibody

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ProSci
Product Code: PSI-79-957
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-957-0.1mg0.1mg£488.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HeLa cells treated with Heatshock using Phospho-PKCB (Ser661) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from HeLa cells treated with Heatshock using Phospho-PKCB (Ser661) Antibody. The lane on the right is treated with the antigen-specific peptide.

Documents

Further Information

Additional Names:
PIM-1, EC 2.7.11.1
Application Note:
Western Blot: 1:500~1:1000
Background:
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1.
Background References:
  • Zhu M, Chen J, Jiang H, Miao C (2013) Cardiovasc Diabetol 12, 13
  • Leonard TA, et al. (2011) Cell 144, 55-66
  • Qiao M, et al. (2010) Mol Cell 38, 512-23
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PKCB (phospho Ser661) antibody was raised against a peptide sequence around phosphorylation site of serine 661 (G-F-S (p)-Y-T) derived from Human PKCB.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5579
NCBI Official Name:
protein kinase C, beta
NCBI Official Symbol:
PRKCB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
NP_002729.2
Protein GI Number:
20127450
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of PKCB only when phosphorylated at serine 661.
Swissprot #:
P05771