Akt (phospho Ser129) Antibody
| Code | Size | Price |
|---|
| PSI-79-966-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
AKT, AKT1 kinase, kinase Akt1, RAC, RAC-PK-alpha
Application Note:
Western Blot: 1:500~1:1000
Background:
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Background References:
- Nguyen le XT, Mitchell BS (2013) Proc Natl Acad Sci U S A 110, 20681-6
- Ponce DP, et al. (2011) Mol Cell Biochem 356, 127-32
- Siddiqui-Jain A, et al. (2010) Cancer Res 70, 10288-98
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Akt (phospho Ser129) antibody was raised against a peptide sequence around phosphorylation site of serine129 (D-N-S (p)-G-A) derived from Human Akt .
Modifications:
Phospho-Specific
NCBI Gene ID #:
207
NCBI Official Name:
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol:
AKT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
NP_001014431.1
Protein GI Number:
62241013
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of Akt only when phosphorylated at serine 129.
Swissprot #:
P31749