mTOR (phospho Thr2446) Antibody

ProSci
Product Code: PSI-79-967
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-967-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from NIH-3T3 cells treated with Insulin using Phospho-mTOR (Thr2446) Antibody. The lane on the right is treated with the antigen-specific peptide.

Western blot analysis of extracts from NIH-3T3 cells treated with Insulin using Phospho-mTOR (Thr2446) Antibody. The lane on the right is treated with the antigen-specific peptide.

Further Information

Additional Names:
FRAP, FRAP1, FRAP2, RAFT1, Rapamycin target protein
Application Note:
Western Blot: 1:500~1:1000
Background:
Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Background References:
  • Holz MK, Blenis J (2005) J Biol Chem 280, 26089-93
  • Cao X, et al. (2005) Mol Endocrinol 19, 102-12
  • Cheng SW, Fryer LG, Carling D, Shepherd PR (2004) J Biol Chem 279, 15719-22
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
mTOR (phospho Thr2446) antibody was raised against a peptide sequence around phosphorylation site of threonine2446 (T-R-T (p)-D-S) derived from Human mTOR .
Modifications:
Phospho-Specific
NCBI Gene ID #:
2475
NCBI Official Name:
mechanistic target of rapamycin (serine/threonine kinase)
NCBI Official Symbol:
MTOR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
220 kDa
Protein Accession #:
NP_004949.1
Protein GI Number:
4826730
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of mTOR only when phosphorylated at threonine 2446.
Swissprot #:
P42345