Smad3 (phospho Ser204) Antibody

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ProSci
Product Code: PSI-79-968
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-968-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from NIH-3T3 cells treated with Serum using Phospho-Smad3 (Ser204) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from NIH-3T3 cells treated with Serum using Phospho-Smad3 (Ser204) Antibody. The lane on the right is treated with the antigen-specific peptide.

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Further Information

Additional Names:
JV15-2, MAD-3, MADH3, Smad 3, Mothers against decapentaplegic homolog 3
Application Note:
Western Blot: 1:500~1:1000
Background:
Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Background References:
  • Cohen-Solal KA, et al. (2011) Pigment Cell Melanoma Res 24, 512-24
  • Seong HA, Jung H, Ha H (2010) J Biol Chem 285, 30959-70
  • Zelivianski S, Cooley A, Kall R, Jeruss JS (2010) Mol Cancer Res 8, 1375-87
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Smad3 (phospho Ser204) antibody was raised against a peptide sequence around phosphorylation site of serine204 (A-G-S (p)-P-N) derived from Human Smad3 .
Modifications:
Phospho-Specific
NCBI Gene ID #:
4088
NCBI Official Name:
SMAD family member 3
NCBI Official Symbol:
SMAD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
NP_001138574.1
Protein GI Number:
223029440
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of Smad3 only when phosphorylated at serine 204.
Swissprot #:
P84022