IKK- beta (phospho Tyr188) Antibody
| Code | Size | Price |
|---|
| PSI-79-980-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK2, IkBKB
Application Note:
Western Blot: 1:500~1:1000
Background:
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Background References:
- Darwech I, Otero JE, Alhawagri MA, Abu-Amer Y (2010)J Biol Chem 285, 25522-30
- Huang WC, Chen JJ, Inoue H, Chen CC (2003) J Immunol 170, 4767-75
- Huang WC, Chen JJ, Chen CC (2003)J Biol Chem 278, 9944-52
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IKK- beta (phospho Tyr188) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 188 (L-Q-Y (p)-L-A) derived from Human IKK- beta.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3551
NCBI Official Name:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
NCBI Official Symbol:
IKBKB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa
Protein Accession #:
NP_001177649.1
Protein GI Number:
14285497
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of IKK- beta only when phosphorylated at tyrosine 188.
Swissprot #:
O14920