IKK- alpha/ beta (phospho Ser180/181) Antibody

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ProSci
Product Code: PSI-79-981
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-981-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HepG2 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser180/181) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from HepG2 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser180/181) Antibody. The lane on the right is treated with the antigen-specific peptide.

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Further Information

Additional Names:
FLJ40509, I-kappa-B kinase, IKBKB, kinase beta, NFKBIKB
Application Note:
Western Blot: 1:500~1:1000
Background:
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
Background References:
  • Hafeez BB, et al. (2008) Cancer Res 68, 8564-72
  • Cahill CM, Rogers JT (2008) J Biol Chem 283, 25900-12
  • Encinas M, et al. (2008)Cell Death Differ 15, 1510-21
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IKK- alpha/ beta (phospho Ser180/181) antibody was raised against a peptide sequence around phosphorylation site of serine 180/181 (C-T-S (p)-F-V) derived from Human IKK- alpha/ beta.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1147
NCBI Official Name:
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol:
CHUK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
NP_001269.3
Protein GI Number:
62241001
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 180/181.
Swissprot #:
O15111/O14920