IKK- alpha/ beta (phospho Ser176/177) Antibody

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ProSci
Product Code: PSI-79-982
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-982-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) Antibody. The lane on the right is treated with the antigen-specific peptide.

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Further Information

Additional Names:
FLJ40509, I-kappa-B kinase, IKBKB, kinase beta, NFKBIKB
Application Note:
Western Blot: 1:500~1:1000
Background:
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.
Background References:
  • Chandrakesan P, et al. (2010)J Biol Chem 285, 33485-98
  • Hinz M, et al. (2010) Mol Cell 40, 63-74
  • Choudhary S, Lu M, Cui R, Brasier AR (2007)Mol Endocrinol 21, 2203-17
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IKK- alpha/ beta (phospho Ser176/177) antibody was raised against a peptide sequence around phosphorylation site of serine 176/177 (Q-G-S (p)-L-C) derived from Human IKK-alpha/beta.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1147
NCBI Official Name:
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol:
CHUK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
NP_001269.3
Protein GI Number:
62241001
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.
Swissprot #:
O15111/O14920