Src (phospho Ser75) Antibody

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ProSci
Product Code: PSI-79-994
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-994-0.1mg0.1mg£488.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from COLO205 tissue using Src (Phospho-Ser75) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from COLO205 tissue using Src (Phospho-Ser75) Antibody. The lane on the right is treated with the antigen-specific peptide.

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Further Information

Additional Names:
p60-Src, c-Src, pp60c-src
Application Note:
Western Blot: 1:500~1:1000
Background:
proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.
Background References:
  • Pan Q, et al. (2011)Cell Mol Life Sci 68, 3425-36 . Kato G, Maeda S (2003) J Biochem (Tokyo) 133, 563-9. Kato G, Maeda S (1999)J Biochem (Tokyo) 126, 957-61.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Src (phospho Ser75) antibody was raised against a peptide sequence around phosphorylation site of serine 75 (V-T-S (p)-P-Q) derived from Human Src.
Modifications:
Phospho-Specific
NCBI Gene ID #:
6714
NCBI Official Name:
v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
NCBI Official Symbol:
SRC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
NP_005408.1
Protein GI Number:
4885609
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of Src only when phosphorylated at serine 75.
Swissprot #:
P12931