CBL (phospho Tyr674) Antibody

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ProSci
Product Code: PSI-79-996
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-996-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HepG2 cells treated with Na2VO3 using Phospho-CBL (Tyr674) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from HepG2 cells treated with Na2VO3 using Phospho-CBL (Tyr674) Antibody. The lane on the right is treated with the antigen-specific peptide.

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Further Information

Additional Names:
C-CBL, CBL2, Proto-oncogene c-Cbl, RNF55,
Application Note:
Western Blot: 1:500~1:1000
Background:
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
Background References:
  • Fernandes MS, et al. (2010) J Biol Chem 285, 32596-605
  • Teckchandani AM, Panetti TS, Tsygankov AY (2005) Exp Cell Res 307, 247-58
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CBL (phospho Tyr674) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine674 (A-I-Y (p)-S-L) derived from Human CBL.
Modifications:
Phospho-Specific
NCBI Gene ID #:
867
NCBI Official Name:
Cas-Br-M (murine) ecotropic retroviral transforming sequence
NCBI Official Symbol:
CBL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
120 kDa
Protein Accession #:
NP_005179.2
Protein GI Number:
115855
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of CBL only when phosphorylated at tyrosine 674.
Swissprot #:
P22681