PKD1/PKC μ (phospho Tyr463) Antibody
| Code | Size | Price |
|---|
| PSI-80-011-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
KPCD1, PKC-mu, PKCM, PKD, PRKD1
Application Note:
Western Blot: 1:500~1:1000
Background:
Serine/threonine-protein kinase that converts transient diacylglycerol
(DAG) signals into prolonged physiological effects downstream of PKC, and is involved in
the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and
trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation
by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling,
and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced
apoptosis and flagellin-stimulated inflammatory response. Phosphorylates the epidermal
growth factor receptor (EGFR) on dual threonine residues, which leads to the
suppression of epidermal growth factor (EGF)-induced MAPK8/JNK1 activation and
subsequent JUN phosphorylation. Phosphorylates RIN1, inducing RIN1 binding to 14-3-3
proteins YWHAB, YWHAE and YWHAZ and increased competition with RAF1 for binding
to GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). Acts downstream of the
heterotrimeric G-protein beta/gamma-subunit complex to maintain the structural
integrity of the Golgi membranes, and is required for protein transport along the
secretory pathway. In the trans-Golgi network (TGN), regulates the fission of transport
vesicles that are on their way to the plasma membrane. May act by activating the lipid
kinase phosphatidylinositol 4-kinase beta (PI4KB) at the TGN for the local synthesis of
phosphorylated inositol lipids, which induces a sequential production of DAG,
phosphatidic acid (PA) and lyso-PA (LPA) that are necessary for membrane fission and
generation of specific transport carriers to the cell surface. Under oxidative stress, is
phosphorylated at Tyr-463 via SRC-ABL1 and contributes to cell survival by activating IKK
complex and subsequent nuclear translocation and activation of NFKB1. Involved in cell
migration by regulating integrin alpha-5/beta-3 recycling and promoting its recruitment
in newly forming focal adhesion.
Background References:
- D?ppler H, Storz P (2007)J Biol Chem 282, 31873-81. Qin L, Zeng H, Zhao D (2006)J Biol Chem 281, 32550-8. Storz P, D?ppler H, Toker A (2004)Mol Pharmacol 66, 870-9 .
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PKD1/PKC µ (phospho Tyr463) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine463 (R-Y-Y (p)-K-E) derived from Human PKD1/PKC µ.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5587
NCBI Official Name:
protein kinase D1
NCBI Official Symbol:
PRKD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
130 kDa
Protein Accession #:
NP_002733.2
Protein GI Number:
115529463
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of PKD1/PKC ? only when phosphorylated at tyrosine 463.
Swissprot #:
Q15139