p70 S6 Kinase (phospho Thr389/412) Antibody

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ProSci
Product Code: PSI-80-023
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-80-023-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from Jurkat cells treated with Insulin using Phospho-p70 S6 Kinase (Thr389/412) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from Jurkat cells treated with Insulin using Phospho-p70 S6 Kinase (Thr389/412) Antibody. The lane on the right is treated with the antigen-specific peptide.

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Further Information

Additional Names:
KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, polypeptide 1
Application Note:
Western Blot: 1:500~1:1000
Background:
Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.
Background References:
  • Hong S, et al. (2014) J Biol Chem
  • Puustinen P, et al. (2014) CJ Cell Biol 204, 713-27
  • Martineau Y, et al. (2014) Mol Cell Biol 34, 1046-53
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p70 S6 Kinase (phospho Thr389/412) antibody was raised against a peptide sequence around phosphorylation site of threonine389/412 (G-F-T (p)-Y-V) derived from Human p70 S6 Kinase .
Modifications:
Phospho-Specific
NCBI Gene ID #:
6198
NCBI Official Name:
ribosomal protein S6 kinase, 70kDa, polypeptide 1
NCBI Official Symbol:
RPS6KB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
NP_001258971.1
Protein GI Number:
4506737
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at tyrosine 389/tyrosine 412.
Swissprot #:
P23443