n-NOS (phospho Ser852) Antibody

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ProSci
Product Code: PSI-80-024
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-80-024-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from A549 tissue using n-NOS (Phospho-Ser852) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from A549 tissue using n-NOS (Phospho-Ser852) Antibody. The lane on the right is treated with the antigen-specific peptide.

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Further Information

Additional Names:
bNOS, Constitutive NOS, IHPS1, N-NOS, NC-NOS
Application Note:
Western Blot: 1:500~1:1000
Background:
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Background References:
  • Capettini LS, et al. (2011) DBr J Pharmacol 164, 1738-48
  • Ferreira JM, Burnett AL, Rameau GA (2011) J Neurosci 31, 1991-9
  • Chaudhury A, Rao YM, Goyal RK (2008) Am J Physiol Gastrointest Liver Physiol 295, G442-51
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
n-NOS (phospho Ser852) antibody was raised against a peptide sequence around phosphorylation site of serine 852 (F-N-S (p)-V-S) derived from Human n-NOS .
Modifications:
Phospho-Specific
NCBI Gene ID #:
4842
NCBI Official Name:
nitric oxide synthase 1 (neuronal)
NCBI Official Symbol:
NOS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
120, 160 kDa
Protein Accession #:
NP_000611.1
Protein GI Number:
10835173
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of n-NOS only when phosphorylated at serine 852.
Swissprot #:
P29475