CDK1/CDC2 (phospho Thr14) Antibody
| Code | Size | Price |
|---|
| PSI-80-045-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
CDC28, CDC2A, CDK1, MPF, kinase Cdc2
Application Note:
Western Blot: 1:500~1:1000
Background:
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.
Background References:
- Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49
- Shreeram S, Hee WK, Bulavin DV (2008) Mol Cell Biol 28, 7442-50
- Mayya V, et al. (2006) Mol Cell Proteomics 5, 1146-57
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CDK1/CDC2 (phospho Thr14) antibody was raised against a peptide sequence around phospho rylation site of threonine14 (E-G-T (p)-Y-G) derived from Human CDK1/CDC2 .
Modifications:
Phospho-Specific
NCBI Gene ID #:
1017
NCBI Official Name:
cyclin-dependent kinase 2
NCBI Official Symbol:
CDK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
NP_001277159.1
Protein GI Number:
16936528
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of CDK1/CDC2 only when phosphorylated at threonine 14.
Swissprot #:
P24941