CaMK2 alpha/beta/delta (phospho Thr305) Antibody
| Code | Size | Price |
|---|
| PSI-79-776-0.1mg | 0.1mg | £488.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
CAMK2A, KCC2A, kinase CaMK2-alpha, CaMKII-alpha
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
Background References:
- Carl W. Tong, J. Physiol., Aug 2004; 558: 927 - 941.
- Pierre R, J. Biol. Chem., Sep 1997; 272: 24133.
- Daliang Wang, PNAS, Jun 1998; 95: 7133.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CaMK2 alpha/beta/delta (phospho Thr305) antibody was raised against a peptide sequence around phosphorylation site of threonine 305 (I-L-T (p)-T-M) derived from Human CaMK2alpha/beta/delta.
Modifications:
Phospho-Specific
NCBI Gene ID #:
816/817
NCBI Official Name:
calcium/calmodulin-dependent protein kinase II beta, calcium/calmodulin-dependent protein kinase II delta
NCBI Official Symbol:
CAMK2B, CAMK2D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
NP_741960.1
Protein GI Number:
25952118
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of CaMKII only when phosphorylated at threonine 305.
Swissprot #:
Q9UQM7