Synaptotagmin (phospho Thr202) Antibody

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ProSci
Product Code: PSI-79-777
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-777-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from colo cells (Lane 2), using Synaptotagmin (Phospho-Thr202) Antibody. The lane on the left is treated with antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Thr202) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from colo cells (Lane 2), using Synaptotagmin (Phospho-Thr202) Antibody. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Thr202) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
LAG, LAP18, STN1, Prosolin, stathmin
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.
Background References:
  • E Carafoli, Crit. Rev. Biochem. Mol. Biol., Apr 2001; 36: 107.
  • Gry Houeland, J Neurophysiol, Jan 2007; 97: 134 - 143.
  • Christelle Carteron, J. Cell Sci., Mar 2006; 119: 898 - 909.
  • Vladimir I. Slepnev, Science, Aug 1998; 281: 821.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Synaptotagmin (phospho Thr202) antibody was raised against a peptide sequence around phosphorylation site of threonine 202 (R-K-T (p)-L-N) derived from Human Synaptotagmin.
Modifications:
Phospho-Specific
NCBI Gene ID #:
6857
NCBI Official Name:
synaptotagmin I
NCBI Official Symbol:
SYT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
NP_001129277.1
Protein GI Number:
5032139
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.
Swissprot #:
P21579