C/EBP-alpha (phospho Ser21) Antibody

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ProSci
Product Code: PSI-79-780
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-780-0.1mg0.1mg£488.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-alpha (phospho-Ser21) Antibody. The lane on the right is treated with the antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-alpha (phospho-Ser21) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-alpha (phospho-Ser21) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-alpha (phospho-Ser21) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
CAP4, ICE8, MACH, MCH5, Mch-5
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Background References:
  • Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.
  • Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.
  • Rapha?l M?tivier, Mol. Endocrinol., Nov 2001; 15: 1953.
  • Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
C/EBP-alpha (phospho Ser21) antibody was raised against a peptide sequence around phosphorylation site of Serine 21 (L-Q-S (p)-P-P) derived from Human C/EBP-alpha.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1050
NCBI Official Name:
CCAAT/enhancer binding protein (C/EBP), alpha
NCBI Official Symbol:
CEBPA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
NP_004355.2
Protein GI Number:
28872794
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at serine 21.
Swissprot #:
P49715