Caspase 9 (phospho Thr125) Antibody

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ProSci
Product Code: PSI-79-781
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-781-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody. The lane on the right is treated with the antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
APAF-3, MCH6, RNCASP9, CASP-9, ICE-LAP6
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Background References:
  • Duan H., J. Biol. Chem. 271:16720-16724(1996).
  • Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
  • Hadano S., Mamm. Genome 10:757-760(1999)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Caspase 9 (phospho Thr125) antibody was raised against a peptide sequence around phosphorylation site of threonine 125 (P-E-T (p)-P-R) derived from Human Caspase 9.
Modifications:
Phospho-Specific
NCBI Gene ID #:
842
NCBI Official Name:
caspase 9, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
NP_001220.2
Protein GI Number:
14790124
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).
Swissprot #:
P55211