p130 Cas (phospho Tyr410) Antibody

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ProSci
Product Code: PSI-79-783
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-783-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas(Phospho-Tyr410) Antibody. The lane on the left is treated with antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas (Phospho-Tyr410) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas(Phospho-Tyr410) Antibody. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas (Phospho-Tyr410) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
BCAR1, CRKAS, CRK-ASSOCIATED SUBSTRATE
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
Background References:
  • Katarzyna Modzelewska, J. Biol. Chem., Dec 2006; 281: 37527 - 37535.
  • Kevin Ogden, Am J Physiol Heart Circ Physiol, Dec 2006; 291: H2857 - H2863.
  • Sangkil Nam, Cancer Res., Oct 2005; 65: 9185 - 9189.
  • Norikazu Yamana, Mol. Cell. Biol., Sep 2006; 26: 6844 - 6
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p130 Cas (phospho Tyr410) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 410 (G-V-Y (p)-A-V) derived from Human p130 Cas .
Modifications:
Phospho-Specific
NCBI Gene ID #:
9564
NCBI Official Name:
breast cancer anti-estrogen resistance 1
NCBI Official Symbol:
BCAR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
130 kDa
Protein Accession #:
NP_055382.2
Protein GI Number:
44662836
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 410.
Swissprot #:
P56945