C-RAF (phospho Tyr341) Antibody
| Code | Size | Price |
|---|
| PSI-79-793-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
C-RAF, C-Raf, CRAF, RAF-1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Background References:
- David R. Hodge, J. Biol. Chem., Jun 1998; 273: 15727.
- Michael A. Beazely, Mol. Pharmacol., Jan 2005; 67: 250 - 259.
- Deanna G. Adams, J. Biol. Chem., Dec 2005; 280: 42644 - 42654.
- Antonino Colanzi, J. Cell Biol., Apr 2003; 161: 27.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
C-RAF (phospho Tyr341) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 341 (S-Y-Y (p)-W-E) derived from Human C-RAF .
Modifications:
Phospho-Specific
NCBI Gene ID #:
5894
NCBI Official Name:
v-raf-1 murine leukemia viral oncogene homolog 1
NCBI Official Symbol:
RAF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
74 kDa
Protein Accession #:
NP_002871.1
Protein GI Number:
4506401
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of Raf1 only when phosphorylated at tyrosine 341.
Swissprot #:
P04049