ITGB4 (phospho Tyr1510) Antibody

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ProSci
Product Code: PSI-79-817
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-817-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HepG2 cells treated with Na2VO3 using ITGB4 (Phospho-Tyr1510) Antibody. The lane on the right is treated with the antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ITGB4 (Phospho-Tyr1510) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from HepG2 cells treated with Na2VO3 using ITGB4 (Phospho-Tyr1510) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ITGB4 (Phospho-Tyr1510) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
GP150, ITB4, CD104 antigen
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility.
Background References:
  • Suzuki S., EMBO J. 9:757-763(1990).
  • Hogervorst F., EMBO J. 9:765-770(1990).
  • Tamura R.N., J. Cell Biol. 111:1593-1604(1990).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ITGB4 (phospho Tyr1510) antibody was raised against a peptide sequence around phosphorylation site of tyrosine1510 (R-D-Y (p)-S-T) derived from Human ITGB4 .
Modifications:
Phospho-Specific
NCBI Gene ID #:
3691
NCBI Official Name:
integrin, beta 4
NCBI Official Symbol:
ITGB4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
202 kDa
Protein Accession #:
NP_000204.3
Protein GI Number:
317373584
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of ITGB4 only when phosphorylated at tyrosine 1510.
Swissprot #:
P16144