c-Jun (phospho Ser63) Antibody

ProSci
Product Code: PSI-79-828
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-828-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

1 / 2
Western blot analysis of extracts from HeLa cells using c-Jun (Phospho-Ser63) Antibody. The lane on the right is treated with the antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using c-Jun (Phospho-Ser63) antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot analysis of extracts from HeLa cells using c-Jun (Phospho-Ser63) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using c-Jun (Phospho-Ser63) antibody (left) or the same antibody preincubated with blocking peptide (right).

Further Information

Additional Names:
AH119, c-Jun, p39, Jun A, AP1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
Background References:
  • Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.
  • Sevilla A, et al. (2004). Oncogene.23(55): 8950-8958.
  • Li L, et al. (2004). J Biol Chem.279(6): 4058-4065.
  • Hurd C, et al. (2002). Oncogene.21(14): 2154-2160.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
c-Jun (phospho Ser63) antibody was raised against a peptide sequence around phosphorylation site of Serine 63 (L-T-S (p)-P-D) derived from Human c-Jun.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3725
NCBI Official Name:
jun proto-oncogene
NCBI Official Symbol:
JUN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
NP_002219.1
Protein GI Number:
4758616
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The Antibody detects endogenous levels of c-Jun only when phosphorylated at Serine 63.
Swissprot #:
P05412