CK-1alpha (phospho Tyr294) Antibody

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ProSci
Product Code: PSI-79-842
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-842-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1alpha (Phospho-Tyr294) Antibody. The lane on the right is treated with the antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1alpha (Phospho-Tyr294) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1alpha (Phospho-Tyr294) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1alpha (Phospho-Tyr294) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
KC1A, CKI-alpha, CSNK1A1, alpha isoform, Casein kinase I
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
Background References:
  • Tapia C., FEBS Lett. 349:307-312(1994).
  • Fish K.J., J. Biol. Chem. 270:14875-14883(1995).
  • Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CK-1alpha (phospho Tyr294) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y (p)-T-F) derived from Human CK-1alpha.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1452/122011
NCBI Official Name:
casein kinase 1, alpha 1, casein kinase 1, alpha 1-like
NCBI Official Symbol:
CSNK1A1, CSNK1A1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
NP_001883.4
Protein GI Number:
68303575
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of CK-1alpha only when phosphorylated at tyrosine 294.
Swissprot #:
P48729/Q8N752