MAP3K1 (phospho Thr1402) Antibody

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ProSci
Product Code: PSI-79-851
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-851-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1402) Antibody. The lane on the left is treated with antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1402) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1402) Antibody. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1402) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
M3K1, MAP3K1, MAPKKK1, MEKK1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
Background References:
  • Schmutz J., Nature 431:268-274(2004).
  • Xia Y., Genes Dev. 12:3369-3381(1998).
  • Vinik B.S., Mamm. Genome 6:782-783(1995)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MAP3K1 (phospho Thr1402) antibody was raised against a peptide sequence around phosphorylation site of threonine 1402 (T-G-A (p)-G-F) derived from Human MAP3K1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4214
NCBI Official Name:
mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
NCBI Official Symbol:
MAP3K1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
130 kDa
Protein Accession #:
NP_005912.1
Protein GI Number:
153945765
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1402.
Swissprot #:
Q13233