PAK1 (phospho Ser204) Antibody

ProSci
Product Code: PSI-79-860
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-860-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from 3T3 cells treated with UV using PAK1 (Phospho-Ser204) Antibody. The lane on the right is treated with the antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot analysis of extracts from 3T3 cells treated with UV using PAK1 (Phospho-Ser204) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody (left) or the same antibody preincubated with blocking peptide (right).

Further Information

Additional Names:
ADRB2, PAK 1, P65-PAK, P68-PAK
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Background References:
  • Brown J.L.,Curr. Biol. 6:598-605(1996).
  • Sells M.A.,Curr. Biol. 7:202-210(1997).
  • The MGC Project Team;Genome Res. 14:2121-2127(2004).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PAK1 (phospho Ser204) antibody was raised against a peptide sequence around phosphorylation site of Serine 204 (T-R-S (p)-V-I) derived from Human PAK1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5058
NCBI Official Name:
p21 protein (Cdc42/Rac)-activated kinase 1
NCBI Official Symbol:
PAK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
NP_002567.3
Protein GI Number:
42794769
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of PAK1 only when phosphorylated at serine 204.
Swissprot #:
Q13153