Ephrin B1/B2/B3 (phospho Tyr324) Antibody

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ProSci
Product Code: PSI-79-890
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-890-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
EPL2, CEL5-L, EFL-3, EFNB1, ELK-L
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
This gene encodes a member of the ephrin family. The encoded protein is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.
Background References:
  • Beckmann M.P., EMBO J. 13:3757-3762(1994).
  • Davis S., Science 266:816-819(1994).
  • Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ephrin B1/B2/B3 (phospho Tyr324) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 324 (G-D-Y (p)-G-H) derived from Human Ephrin B1/B2/B3 .
Modifications:
Phospho-Specific
NCBI Gene ID #:
1947/1948/1949
NCBI Official Name:
ephrin-B1, ephrin-B2, ephrin-B3
NCBI Official Symbol:
EFNB1, EFNB2, EFNB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
NP_004420.1
Protein GI Number:
4758248
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324.
Swissprot #:
P98172/P52799/Q15768