PLD2 (phospho Tyr169) Antibody

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ProSci
Product Code: PSI-79-922
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-922-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
PLD 2, PLD1C, choline phosphatase 2
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.
Background References:
  • Steed P.M., FASEB J. 12:1309-1317(1998).
  • Lopez I., J. Biol. Chem. 273:12846-12852(1998).
  • Divecha N., EMBO J. 19:5440-5449(2000).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PLD2 (phospho Tyr169) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 169 (E-N-Y (p)-L-N) derived from Human PLD2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5338
NCBI Official Name:
phospholipase D2
NCBI Official Symbol:
PLD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
95 kDa
Protein Accession #:
NP_002654.3
Protein GI Number:
156564407
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
Swissprot #:
O14939