PKA alpha/beta CAT (phospho Thr197) Antibody

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ProSci
Product Code: PSI-79-792
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-792-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from mouse brain cells using PKA alpha/beta CAT (Phospho-Thr197) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from mouse brain cells using PKA alpha/beta CAT (Phospho-Thr197) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
PRKACA , PRKACB, PKACA
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
Background References:
  • Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259.
  • W Li, Learn. Mem., Jan 1996; 2: 320 - 333.
  • Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.
  • Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p)-T-L) derived from Human PKA alpha/beta CAT.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5566
NCBI Official Name:
protein kinase, cAMP-dependent, catalytic, alpha
NCBI Official Symbol:
PRKACA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
NP_002721.1
Protein GI Number:
4506055
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.
Swissprot #:
P17612