CD8a Antibody [53-6.7] (APC-Cy7)
| Code | Size | Price |
|---|
| PSI-76-698-0.1mg | 0.1mg | £433.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: Rat IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 53-6.7
Regulatory Status: RUO
Target Species: Mouse
Application: Flow Cytometry
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Documents
Further Information
Additional Names:
Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, Cd8a
Background:
The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha? chain, of the mouse CD8 antigen. The alpha? chain is the truncated form of alpha chain, encoded by the same CD8a gene. In CD8a, the alpha and alpha? chains form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b. CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. Its function seems to be to attenuate the CD8-mediated signal for the stimulation of intrathymic T-cell maturation.The 53-6.7 antibody is useful for depleting CD8+ peripheral T lymphocytes. It cross reacts with the alpha- and alpha?-like polypeptides on some thymic and peripheral lymphocytes.
Background References:
- Knapp W(1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.
- Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
APC-Cy7
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
12525
NCBI Official Name:
CD8 antigen, alpha chain
NCBI Official Symbol:
Cd8a
NCBI Organism:
Mus musculus
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology
SPECIFICITY:
The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha? chain, of the mouse CD8 antigen.
Swissprot #:
P01731
User NOte:
Optimal dilutions for each application to be determined by the researcher.