CD3e Antibody [145-2C11] (PerCP-Cy5.5)
| Code | Size | Price |
|---|
| PSI-76-274-0.025mg | 0.025mg | £307.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Hamster
Antibody Isotype: Armenian Hamster IgG
Antibody Clonality: Monoclonal
Antibody Clone: 145-2C11
Regulatory Status: RUO
Target Species: Mouse
Application: Flow Cytometry
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Documents
Further Information
Additional Names:
CD3, T3e, AI504783, CD3epsilon, Cd3e
Background:
The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa epsilon chain of the TCR complex. Together with the gamma and delta subunits of CD3, the epsilon subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the epsilon chain enhances the antigen recognition.The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes. The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.
Background References:
- Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., Bluestone, J. A. (1987). Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proceedings of the National Academy of Sciences,84(5), 1374-1378.
- Nakano, H., Yamazaki, T., Miyatake, S., Nozaki, N., Kikuchi, A., Saito, T. (1996). Specific Interaction of Topoisomerase II and the CD3 Chain of the T Cell Receptor Complex.Journal of Biological Chemistry,271(11), 6483-6489.
- Salmond, R. J., Filby, A., Pirinen, N., Magee, A. I., Zamoyska, R. (2011). Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas.Blood,117(1), 108-117.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
PerCP-Cy5.5
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
12501
NCBI Official Name:
CD3 antigen, epsilon polypeptide
NCBI Official Symbol:
Cd3e
NCBI Organism:
Mus musculus
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology
SPECIFICITY:
The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa epsilon chain of the TCR complex.
Swissprot #:
P22646
User NOte:
Optimal dilutions for each application to be determined by the researcher.