CD16 Antibody [2.4G2]

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ProSci
Product Code: PSI-76-573
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-76-573-1mg1mg£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: Rat IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2.4G2
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Flow Cytometry
  • Functional Study
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Documents

Further Information

Additional Names:
Fcgr3, Fcgr3
Background:
The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc gamma III) and CD 32 (Fc gamma II). CD16 and CD32 are low affinity receptors for the IgG Fc domain and are expressed by B lymphocytes, NK cells, kupffer cells, mast cells, monocytes, macrophages, granulocytes, immature thymocytes, neutrophils, and some activated mature T cells.The 2.4G2 antibody blocks the binding of immunoglobulins to CD16 and CD32, and possibly to Fc gamma I receptor.
Background References:
  • Araujo-Jorge, T. A. N. I. A., Rivera, M. T., el Bouhdidi, A. Y. A. C. H. I., Daron, M. A. R. C., Carlier, Y. (1993). An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4 G2) decreases acute Trypanosoma cruzi infection in mice.Infection and immunity,61(11), 4925-4928.
  • Jensen, W. A., Marschner, S., Ott, V. L., Cambier, J. C. (2001). FcgammaRIIB-mediated inhibition of T-cell receptor signal transduction involves the phosphorylation of SH2-containing inositol 5-phosphatase (SHIP), dephosphorylation of the linker of activated T-cells (LAT) and inhibition of calcium mobilization.Biochemical Society Transactions,29(Pt 6), 840-846.
  • Vremec, D., Zorbas, M., Scollay, R., Saunders, D. J., Ardavin, C. F., Wu, L., Shortman, K. (1992). The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells.The Journal of experimental medicine,176(1), 47-58.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
14131
NCBI Official Name:
Fc receptor, IgG, low affinity III
NCBI Official Symbol:
Fcgr3
NCBI Organism:
Mus musculus
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Innate Immunity
SPECIFICITY:
The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc gamma III) and CD 32 (Fc gamma II).
Swissprot #:
P08508
User NOte:
Optimal dilutions for each application to be determined by the researcher.