CD45 Antibody [30-F11]

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ProSci
Product Code: PSI-76-523
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-76-523-0.1mg0.1mg£348.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: Rat IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 30-F11
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Flow Cytometry
  • Functional Study
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Documents

Further Information

Additional Names:
loc, B220, Cd45, L-CA, Ly-5, T200, CD45R, Lyt-4, Ptprc
Background:
The 30-F11 monoclonal antibody specifically reacts with all isoforms of CD45 and also with the alloantigens CD45.1 and CD45.2 (LCA). CD45 is a transmembrane glycoprotein, expressed by all the hematopoietic cells, except for platelets and mature erythrocytes, which distinguishes the leukocytes from the non-hematopoietic cells. The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region?s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons.The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction.
Background References:
  • Ledbetter, J. A., Herzenberg, L. A. (1979). Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*.Immunological reviews,47(1), 63-90.
  • Thomas, M. L. (1989). The leukocyte common antigen family.Annual review of immunology,7(1), 339-369.
  • Simon, D. I., Chen, Z., Seifert, P., Edelman, E. R., Ballantyne, C. M., Rogers, C. (2000). Decreased neointimal formation in Mac-1/mice reveals a role for inflammation in vascular repair after angioplasty.Journal of Clinical Investigation,105(3), 293-300.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
19264
NCBI Official Name:
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol:
Ptprc
NCBI Organism:
Mus musculus
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology,Functional Assays
SPECIFICITY:
The 30-F11 monoclonal antibody specifically reacts with all isoforms of CD45 and also with the alloantigens CD45.1 and CD45.2 (LCA).
Swissprot #:
P06800
User NOte:
Optimal dilutions for each application to be determined by the researcher.