CD279 Antibody [J43.1]

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ProSci
Product Code: PSI-76-975
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-76-975-0.1mg0.1mg£373.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Hamster
Antibody Isotype: Armenian Hamster IgG
Antibody Clonality: Monoclonal
Antibody Clone: J43.1
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Flow Cytometry
  • Functional Study
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Documents

Further Information

Additional Names:
PD-1, Pdc1, Ly101, Pdcd1
Background:
The J43.1 monoclonal antibody specifically reacts with mouse CD279, also known as PD-1 (programmed death-1), a 50-55 kDa glycoprotein of the Ig superfamily. The PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-H2) are members of the B7 family. Pd-1 contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) and influences the peripheral tolerances and autoimmune diseases in mice. PD-1 is transiently expressed on CD4/CD8 thymocytes, it is upregulated in apoptotic cells, and it is expressed by activated myeloid and T and B cells. The binding of PD-1 to its ligands is blocked by the J43 antibody, which also enhances contact hypersensitivity and exacerbates acute Graft-versus-host disease, Experimental autoimmune encephalomyelitis and NOD diabetes. PD-1 seems to downregulate the immune response, as the development of splenomegaly and breakdown of peripheral tolerance in PD-1 deficient mice suggests.
Background References:
  • Nishimura, H., Agata, Y., Kawasaki, A., Sato, M., Imamura, S., Minato, N., ... Honjo, T. (1996). Developmentally regulated expression of the PD-1 protein on the surface of double-negative (CD4CD8) thymocytes.International immunology,8(5), 773-780.
  • Salama, A. D., Chitnis, T., Imitola, J., Ansari, M. J. I., Akiba, H., Tushima, F., ... Khoury, S. J. (2003). Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis.The Journal of experimental medicine,198(1), 71-78.
  • Carreno, B. M., Collins, M. (2002). The B7 family of ligands and its receptors: new pathways for costimulation and inhibition of immune responses.Annual review of immunology,20(1), 29-53.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
18566
NCBI Official Name:
programmed cell death 1
NCBI Official Symbol:
Pdcd1
NCBI Organism:
Mus musculus
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology,Apoptosis,Functional Assays
SPECIFICITY:
The J43.1 monoclonal antibody specifically reacts with mouse CD279, also known as PD-1 (programmed death-1), a 50-55 kDa glycoprotein of the Ig superfamily.
Swissprot #:
Q02242
User NOte:
Optimal dilutions for each application to be determined by the researcher.