CD45.1 Antibody [A20]
| Code | Size | Price |
|---|
| PSI-76-535-0.1mg | 0.1mg | £307.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: A20
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Documents
Further Information
Additional Names:
loc, B220, Cd45, L-CA, Ly-5, T200, CD45R, Lyt-4, Ptprc
Background:
The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
Background References:
- Shen, F.W. (1981) Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. G.J. H U. H and J.F. Kearney, editors. Elsevier/North-Holland, Amsterdam. pp. 2531.
- Yakura, H. I. D. E. T. A. K. A., Kawabata, I., Shen, F. W., Katagiri, M. (1986). Selective inhibition of lipopolysaccharide-induced polyclonal IgG response by monoclonal Ly-5 antibody.The Journal of Immunology,136(8), 2729-2733.
- Yakura, H., Shen, F. W., Bourcet, E., Boyse, E. A. (1983). On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2.The Journal of experimental medicine,157(4), 1077-1088.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
19264
NCBI Official Name:
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol:
Ptprc
NCBI Organism:
Mus musculus
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology
SPECIFICITY:
The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA).
Swissprot #:
P06800
User NOte:
Optimal dilutions for each application to be determined by the researcher.