CD4 Antibody [RM4-5] (Violet-450)
| Code | Size | Price |
|---|
| PSI-76-371-0.025mg | 0.025mg | £307.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: Rat IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: RM4-5
Regulatory Status: RUO
Target Species: Mouse
Application: Flow Cytometry
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Documents
Further Information
Additional Names:
L3T4, Ly-4, Cd4
Background:
The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck).Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
Background References:
- Shevach, E. M. (2000). Regulatory T cells in autoimmmunity*.Annual review of immunology,18(1), 423-449.
- Wu, L., Scollay, R., Egerton, M., Pearse, M., Spangrude, G. J., Shortman, K. (1991). CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus.
- Bliss, S. K., Bliss, S. P., Beiting, D. P., Alcaraz, A., Appleton, J. A. (2007). IL-10 regulates movement of intestinally derived CD4+ T cells to the liver.The Journal of Immunology,178(12), 7974-7983.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
Violet-450
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
12504
NCBI Official Name:
CD4 antigen
NCBI Official Symbol:
Cd4
NCBI Organism:
Mus musculus
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology
SPECIFICITY:
The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells.
Swissprot #:
P06332
User NOte:
Optimal dilutions for each application to be determined by the researcher.