CD8a Antibody [2.43] (Violet-450)
| Code | Size | Price |
|---|
| PSI-76-648-0.025mg | 0.025mg | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: Rat IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2.43
Regulatory Status: RUO
Target Species: Mouse
Application: Flow Cytometry
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Documents
Further Information
Additional Names:
Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, Cd8a
Background:
The 2.43 monoclonal antibody specifically reacts with mouse CD8 antigen. CD8a (the alpha chains) form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b. CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
Background References:
- Salem, M. L., Hossain, M. S. (2000). In vivo acute depletion of CD8+ T cells before murine cytomegalovirus infection upregulated innate antiviral activity of natural killer cells. International journal of immunopharmacology,22(9), 707-718.
- Kruisbeek, A. M. (1991). In Vivo Depletion of CD4 and CD8 Specific T Cells.Current protocols in immunology, 4-1.
- Davies, A., Kalb, S., Liang, B., Aldrich, C. J., Lemonnier, F. A., Jiang, H., ... Soloski, M. J. (2003). A peptide from heat shock protein 60 is the dominant peptide bound to Qa-1 in the absence of the MHC class Ia leader sequence peptide Qdm.The Journal of Immunology,170(10), 5027-5033.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
Violet-450
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
12525
NCBI Official Name:
CD8 antigen, alpha chain
NCBI Official Symbol:
Cd8a
NCBI Organism:
Mus musculus
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology
SPECIFICITY:
The 2.43 monoclonal antibody specifically reacts with mouse CD8 antigen.
Swissprot #:
P01731
User NOte:
Optimal dilutions for each application to be determined by the researcher.