Ras-GRF1 (phospho Ser916) Antibody
| Code | Size | Price |
|---|
| PSI-79-801-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Mouse
- Rat
Application: Immunohistochemistry (IHC)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
CDC25, GNRP, GRF1, RASGRF1
Background:
Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.
Background References:
- Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.
- Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.
- Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.
- Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ras-GRF1 (phospho Ser916) antibody was raised against a peptide sequence around phosphorylation site of Serine 916 (N-E-G (p)-T P) derived from Human Ras-GRF1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
19417
NCBI Official Name:
RAS protein-specific guanine nucleotide-releasing factor 1
NCBI Official Symbol:
Rasgrf1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
145 kDa
Protein Accession #:
NP_002882.3
Protein GI Number:
6755288
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.
Swissprot #:
Q13972