CD40 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-089-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Background References:
- Banchereau, J. et al., 1994, Annu. Rev. Immunol. 12: 881-922.
- Ni, C.Z. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 10395-10399.
- Ferrari.S. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 12614-12619.
- Bhushan, A and Covey, L.R. 2001, Immunol. Res. 24: 311-324.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized rhCD40-Fc at 2μg/ml (100μl/well) can bind biotinylated human CD40L with a linear range of 7. 8 - 125 ng/mL.
Buffer:
Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5
Fusion Tag:
C-Fc Tag
NCBI Gene ID #:
958
NCBI Official Name:
CD40 molecule, TNF receptor superfamily member 5
NCBI Official Symbol:
CD40
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
45.3 kDa
Protein Accession #:
NP_001241.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P25942