CD86 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-127-0.2mg | 0.2mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CD86, B7-2, B70, CD28LG2, LAB72, MGC34413
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
Background References:
- Chen C, et al., 1994, J. Immunol. 152 (10): 4929?36.
- Yadav, D. et al., 2007, J. Immunol. 178: 6236-6241.
- Steidl C, et al., 2010, N. Engl. J. Med. 362 (10): 875?85.
Biological Activity:
Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0. 04 - 0. 2 ng/ml. Measured by its binding ability in a functional ELISA. Immobilized rhB7-2 / CD86 Fc Chimera at 2μg/mL (100μL/well) can bind Biotinylated rh CTLA4, His. Avi Tag with a linear range of 0. 1 - 1μg/mL.
Buffer:
Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5
Fusion Tag:
C-Fc Tag
NCBI Gene ID #:
942
NCBI Official Name:
CD86 molecule
NCBI Official Symbol:
CD86
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
51.5 kDa
Protein Accession #:
AAH40261
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P42081