GCSFR Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-342-0.1mg | 0.1mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CSF3R, CD114, GCSFR
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Granulocyte Colony Stimulating Factor Receptor (G-CSFR) is also known as Cluster of Differentiation 114 (CD114), CSF3R and GCSF, is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF), a cytokine that plays a critical role in the regulation of the activation, proliferation, differentiation, and survival of the neutrophilic granulocyte lineage. G-CSFR belongs to a family of cytokine receptors known as the hematopoietin receptor family. This type I? membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type I?II (FNIII) domains in the extracellular region. G-CSFR is present mainly on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. G-CSFR mediates the specific effect of GCSF through activating a variety of intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways, and thus functions in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. Mutations in the intracellular part of this receptor are also associated with certain types of leukemia.
Background References:
- Liu H, et al., 2006, J Immunol, 176(4):2407-13.
- Zeidler C, Welte K, 2002, Semin. Hematol. 39 (2): 82?8.
- Beekman R, et al., 2010, Blood, 115(25): 5131-6.
Biological Activity:
Measured by its ability to inhibit the GCSF-induced proliferation of NFS-60 mouse myeloid cells. The ED50 for this effect is typically 0. 02-2μg /ml in the presence of 0. 125 ng /ml of recombinant human GCSF.
Buffer:
PBS, pH7.4
Fusion Tag:
C-His Tag
NCBI Gene ID #:
1441
NCBI Official Name:
colony stimulating factor 3 receptor (granulocyte)
NCBI Official Symbol:
CSF3R
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
69 kDa
Protein Accession #:
NP_000751.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q99062