AKT1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-020-0.025mg | 0.025mg | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
AKT1, PKB, RAC, RAC-PK-alpha, PKB alpha
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
RAC-alpha serine/threonine-protein kinase (AKT1) is also known PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt and RAC-PK-alpha, which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily and is expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase).
Background References:
- Jones P.F., et al., 1991, Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175.
- Goshima N., et al., 2008 Nat. Methods 5:1011-1017.
- Delcommenne M., et al ., 1998, Proc. Natl. Acad. Sci. U.S.A. 95:11211-11216.
Buffer:
20 mM tris, 150 mM NaCl, pH8.0
Fusion Tag:
C-His, C-Twin Strep Tag
NCBI Gene ID #:
207
NCBI Official Name:
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol:
AKT1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
59.5 kDa
Protein Accession #:
AAH00479
Purity:
>92% as determined by reduced SDS-PAGE.
Less than 1.0 EU per ug by the LAL method.
Less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P31749