Transferrin Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-755-0.2mg | 0.2mg | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Transferrin, TF, DKFZp781D0156, PRO1557, PRO2086
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Transferrin is also known as Serotransferrin, Beta-1 metal-binding globulin, TF, and is iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. Although iron bound to transferrin is less than 0.1% (4?mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25?mg/24 h). The affinity of transferrin for Fe(III) is extremely high (1023 M1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.When not bound to iron, it is known as "apo-transferrin?. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. It is a complex composed of alpha helices and beta sheets to form two domains (the first situated in the N-terminus and the second in the C-terminus). The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. The liver is the main source of manufacturing transferrin, but other sources such as the brain also produce this molecule . Transferrin is also associated with the innate immune system. Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron that impedes bacteria survival in a process called iron withholding. The level of transferrin decreases in inflammation. The metal binding properties of transferrin have a great influence on the biochemistry of plutonium in humans. Transferrin has a bacteriocidal effect on bacteria, in that it makes Fe3+ unavailable to the bacteria.Carbohydrate deficient transferrin increases in the blood with heavy ethanol consumption and can be monitored via laboratory testing.
Background References:
- Crichton RR, Charloteaux-Wauters M. 1987, Eur. J. Biochem. 164 (3): 485?506.
- Aisen, Phillip, et al.,1978, Journal of Biological Chemistry 253 (6): 1930?1937.
- Ritchie RF,et al., 1999, J. Clin. Lab. Anal. 13 (6): 273?9.
- Sharpe PC, 2001, Ann. Clin. Biochem. 38 (Pt 6): 652?64.
Biological Activity:
Measured by its binding ability in a functional ELISA.Immobilized Human Transferrin, His Tag at 10 μg/ml (100 μL/well),can bind Biotinylated Human Transferrin R with a linear range of 2-200 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
7018
NCBI Official Name:
transferrin
NCBI Official Symbol:
TF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
76 kDa
Protein Accession #:
AAH59367
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P02787