Carbonic Anhydrase 2 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-072-0.1mg | 0.1mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CA2, CA-II, CAII, Car2
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore, classified as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ and ε). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The α-CAs are found in humans.
Carbonic anhydrase II (CA2) is also known as Carbonate dehydratase II, Carbonic anhydrase C, is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
Background References:
- Badger MR, Price GD, 1994, Annu. Rev. Plant Physiol. Plant Mol. Bio. 45: 369?392.
- Nair, S. K. et al., 1991, The Journal of biological chemistry 266 (26): 17320?17325.
- Sterling, D., et al., 2001, J. Biol. Chem. (United States) 276 (51): 47886?94.
- Vince, J W., et al., 2000, Biochemistry (UNITED STATES) 39 (44): 13344?9.
Biological Activity:
Measured by its esterase activity for digestion of 4?Nitrophenyl?Acetate(4?NPA). The specific activity is > 150 pmoles/min/μg.
Buffer:
20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT.
Fusion Tag:
His Tag
NCBI Gene ID #:
760
NCBI Official Name:
carbonic anhydrase II
NCBI Official Symbol:
CA2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
30 kDa
Protein Accession #:
NP_000058.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P00918